Publikace
Vybrané vědecké publikace na téma potápěčské medicíny a fyziologie
2021 Čvc 26
Heart Rate Variability during a standard dive: a role for inspired oxygen pressure?
Pierre Lafère, Kate Lambrechts, Peter Germonpre, Ambre Balestra, Faye Lisa Germonpré, Alessandro Marroni, Danilo Cialoni, Gerardo Bosco, Costantino Balestra

Introduction: Heart rate variability (HRV) during underwater diving has been infrequently investigated because of environment limitations and technical challenges. This study aims to analyze HRV changes while diving at variable hyperoxia when using open circuit (OC) air diving apparatus or at constant hyperoxia using a closed-circuit rebreather (CCR).

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2018 Srp 21
Hemoglobin and Erythropoietin After Commercial Saturation Diving
Kiboub FZ., Balestra C., Loennechen Ø., Eftedal I.

Saturation divers are exposed to elevated partial pressure of oxygen (ppO2) in their hyperbaric work environment. Experimental studies indicate that oxygen transport is altered, and we have previously reported a drop in hematocrit and extensive downregulation of genes involved in blood oxygen transport capacity after decompression from professional saturation diving. Here we investigate the initial period of hematological adjustment back to normobaric air after professional saturation diving. Erythropoietin (EPO) and hemoglobin (Hb) were measured in blood from 13 divers at two time-points after saturation assignments lasting up to 4 weeks; first immediately after decompression and again 24 h later. Pre-dive levels defined baselines.

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2021 Čvc 28
High-Throughput Griess Assay of Nitrite and Nitrate in Plasma and Red Blood Cells for Human Physiology Studies under Extreme Conditions
Brizzolari, A.; Dei Cas, M.; Cialoni, D.; Marroni, A.; Morano, C.; Samaja, M.; Paroni, R.; Rubino, F.M.

The metabolism of nitric oxide plays an increasingly interesting role in the physiological response of the human body to extreme environmental conditions, such as underwater, in an extremely cold climate, and at low oxygen concentrations.

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2020 Úno 12
Hyperoxia Alters Ultrastructure and Induces Apoptosis in Leukemia Cell Lines
David De Bels, Frauke Tillmans, Francis Corazza, Mariano Bizzarri, Peter Germonpre, Peter Radermacher, Keziban Günce Orman, and Costantino Balestra

Oxygenation conditions are crucial for growth and tumor progression. Recent data suggests a decrease in cancer cell proliferation occurring after exposure to normobaric hyperoxia. Those changes are associated with fractal dimension. The purpose of this research was to study the impact of hyperoxia on apoptosis and morphology of leukemia cell lines. Two hematopoietic lymphoid cancer cell lines (a T-lymphoblastoid line, JURKAT and a B lymphoid line, CCRF-SB) were tested under conditions of normobaric hyperoxia (FiO2 > 60%, ± 18h) and compared to a standard group (FiO2 = 21%). We tested for apoptosis using a caspase-3 assay.

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2021 Zář 4
Hypoxic and Hyperoxic Breathing as a Complement to Low-Intensity Physical Exercise Programs: A Proof-of-Principle Study
Balestra C, Lambrechts K, Mrakic-Sposta S, Vezzoli A, Levenez M, Germonpre P, Virgili F, Bosco G, Lafere P

Inflammation is an adaptive response to both external and internal stimuli including infection, trauma, surgery, ischemia-reperfusion, or malignancy. A number of studies indicate that physical activity is an effective means of reducing acute systemic and low-level inflammation occurring in different pathological conditions and in the recovery phase after disease. As a proof-of-principle, we hypothesized that low-intensity workout performed under modified oxygen supply would elicit a “metabolic exercise” inducing a hormetic response, increasing the metabolic load and oxidative stress with the same overall effect expected after a higher intensity or charge exercise.

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